Research articles

2016

  • Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A.

  • A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative.
  • Sci Rep. 2016 May 31;6;26141. doi:10.1038/srep26141
  • PubMed Abstract Full Text
  • Green B, Kovalev S, Asgekar V, Geloni G, Lehnert U, Golz T, Kuntzsch M, Bauer C, Hauser J, Voigtlaender J, Wustmann B, Koesterke I, Schwarz M, Freitag M, Arnold A, Teichert J, Justus M, Seidel W, Ilgner C, Awari N, Nicoletti D, Kaiser S, Laplace Y, Rajasekaran S, Zhang L, Winnerl S, Schneider H, Schay G, Lorincz I, Rauscher AA, Radu I, Mährlein S, Kim TH, Lee JS, Kampfrath T, Wall S, Heberle J, Málnási-Csizmadia A.

    Steiger A, Müller AS, Helm M, Schramm U, Cowan T, Michel P, Cavalleri A, Fisher AS, Stojanovic N, Gensch M.

  • High-Field High-Repetition-Rate Sources for the Coherent THz Control of Matter.
  • Sci Rep. 2016 Feb 29;6:22256. doi: 1038/srep22256
  • PubMed Abstract Full Text

2015

  • Képiró M, Várkuti BH, Rauscher AA, Kellermayer MS, Varga M, Málnási-Csizmadia A.
  • Molecular Tattoo: Subcellular Confinement of Drug Effects.
  • Chem Biol. 2015 Apr 16. pii: S1074-5521(15)00106-4. doi: 10.1016/j.chembiol.2015.03.013. [Epub ahead of print]
  • PubMed Abstract Full Text

2014

  • Képiró M, Várkuti BH, Végner L, Vörös G, Hegyi G, Varga M, Málnási-Csizmadia A.
  • para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor.
  • Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. doi: 10.1002/anie.201403540.
  • PubMed Abstract Full Text
  • Swenson AM, Trivedi DV, Rauscher AA, Wang Y, Takagi Y, Palmer BM, Málnási-Csizmadia A, Debold EP, Yengo CM.
  • Magnesium modulates actin binding and ADP release in myosin motors.
  • J Biol Chem. 2014 Aug 22;289(34):23977-91. doi: 10.1074/jbc.M114.562231. Epub 2014 Jul 8.
  • PubMed Abstract Full Text

2013

  • Peragovics A, Simon Z, Tombor L, Jelinek B, Hári P, Czobor P, Málnási-Csizmadia A.
  • Virtual Affinity Fingerprints for Target Fishing: A New Application of Drug Profile Matching.
  • J Chem Inf Model. 2013 Jan 28;53(1):103-13. doi: 10.1021/ci3004489.
  • PubMed Abstract Full Text
  • Végner L, Peragovics Á, Tombor L, Jelinek B, Czobor P, Bender A, Simon Z, Málnási-Csizmadia A.
  • Experimental confirmation of new drug-target interactions predicted by Drug Profile Matching.
  • J Med Chem. 2013 Nov 14;56(21):8377-88. doi: 10.1021/jm400813y.
  • PubMed Abstract Full Text
  • Kovács D, Simon Z, Hári P, Málnási-Csizmadia A, Heged?s C, Drimba L, Németh J, Sári R, Szilvássy Z, Peitl B.
  • Identification of PPARγ ligands with One-dimensional Drug Profile Matching.
  • Drug Des Devel Ther. 2013 Sep 2;7:917-28. doi: 10.2147/DDDT.S47173.
  • PubMed Abstract Full Text

2012

  • Simon Z, Peragovics A, Vigh-Smeller M, Csukly G, Tombor L, Yang Z, Zahoránszky-K?halmi G, Végner L, Jelinek B, Hári P, Hetényi C, Bitter I, Czobor P, Málnási-Csizmadia A.
  • Drug effect prediction by polypharmacology-based interaction profiling
  • J Chem Inf Model. 2012 Jan 23;52(1):134-45. doi: 10.1021/ci2002022.
  • E-mail reprint request PubMed Abstract Full Text
  • Várkuti BH, Yang Z, Kintses B, Erdélyi P, Bárdos-Nagy I, Kovács AL, Hári P, Kellermayer M, Vellai T, Málnási-Csizmadia A.
  • A novel actin binding site of myosin required for effective muscle contraction.
  • Nat Struct Mol Biol. 2012 Feb 12;19(3):299-306. doi: 10.1038/nsmb.2216.
  • E-mail reprint request PubMed Abstract Full Text
  • Képiró M, Várkuti BH, Bodor A, Hegyi G, Drahos L, Kovács M, Málnási-Csizmadia A.
  • Azidoblebbistatin, a photoreactive myosin inhibitor.
  • Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9402-7. doi: 10.1073/pnas.1202786109.
  • E-mail reprint request PubMed Abstract Full Text
  • Peragovics A, Simon Z, Brandhuber I, Jelinek B, Hári P, Hetényi C, Czobor P, Málnási-Csizmadia A.
  • Contribution of 2D and 3D Structural Features of Drug Molecules in the Prediction of Drug Profile Matching.
  • J Chem Inf Model. 2012 Jun 29. [Epub ahead of print]
  • E-mail reprint request PubMed Abstract Full Text

2011

  • Purcell TJ, Naber N, Franks-Skiba K, Dunn AR, Eldred CC, Berger CL, Málnási-Csizmadia A, Spudich JA, Swank DM, Pate E, Cooke R.
  • Nucleotide pocket thermodynamics measured by EPR reveal how energy partitioning relates myosin speed to efficiency.
  • J Mol Biol. 2011 Mar 18;407(1):79-91. Epub 2010 Dec 23.
  • E-mail reprint request PubMed Abstract Full Text

2010

  • Simon Z, Vigh-Smeller M, Peragovics A, Csukly G, Zahoránszky-Kohalmi G, Rauscher AA, Jelinek B, Hári P, Bitter I, Málnási-Csizmadia A, Czobor P.
  • Relating the shape of protein binding sites to binding affinity profiles: is there an association?
  • BMC Struct Biol. 2010 Oct 5;10:32. doi: 10.1186/1472-6807-10-32.
  • PubMed Abstract Full Text
  • Takács B, Billington N, Gyimesi M, Kintses B, Málnási-Csizmadia A, Knight PJ, Kovács M.
  • Myosin complexed with ADP and blebbistatin reversibly adopts a conformation resembling the start point of the working stroke.
  • Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6799-804. Epub 2010 Mar 29.
  • PubMed Abstract Full Text

2009

  • Zahoránszky LA, Katona GY, Hári P, Málnási-Csizmadia A, Zweig KA, Zahoránszky-Köhalmi G.
  • Breaking the hierarchy — a new cluster selection mechanism for hierarchical clustering methods.
  • Algorithms Mol Biol. 2009 Oct 19;4:12.
  • PubMed Abstract Full Text

2008

  • Gyimesi M, Kintses B, Bodor A, Perczel A, Fischer S, Bagshaw CR, Málnási-Csizmadia A.
  • The mechanism of the reverse recovery-step, phosphate release, and actin activation of Dictyostelium myosin II.
  • J Biol Chem. 2008 Mar 28;283(13):8153-63. Epub 2008 Jan 21.
  • PubMed Abstract Full Text
  • Gombos L, Kardos J, Patthy A, Medveczky P, Szilagyi L, Malnasi-Csizmadia A, Graf L.
  • Probing conformational plasticity of the activation domain of trypsin: the role of glycine hinges.
  • Biochemistry. 2008 Feb 12;47(6):1675-84. Epub 2008 Jan 15.
  • E-mail reprint request PubMed Abstract Full Text

2007

  • Tóth J, Varga B, Kovács M, Málnási-Csizmadia A, Vértessy BG.
  • Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme.
  • J Biol Chem. 2007 Nov 16;282(46):33572-82. Epub 2007 Sep 11.
  • E-mail reprint request PubMed Abstract Full Text
  • Málnási-Csizmadia A, Tóth J, Pearson DS, Hetényi C, Nyitray L, Geeves MA, Bagshaw CR, Kovács M.
  • Selective perturbation of the myosin recovery stroke by point mutations at the base of the lever arm affects ATP hydrolysis and phosphate release.
  • J Biol Chem. 2007 Jun 15;282(24):17658-64. Epub 2007 Apr 21.
  • PubMed Abstract Full Text
  • Tóth J, Simon Z, Medveczky P, Gombos L, Jelinek B, Szilágyi L, Gráf L, Málnási-Csizmadia A.
  • Site directed mutagenesis at position 193 of human trypsin 4 alters the rate of conformational change during activation: role of local internal viscosity in protein dynamics.
  • Proteins. 2007 Jun 1;67(4):1119-27.
  • E-mail reprint request PubMed Abstract Full Text

2006

  • Kintses B, Simon Z, Gyimesi M, Tóth J, Jelinek B, Niedetzky C, Kovács M, Málnási-Csizmadia A.
  • Enzyme kinetics above denaturation temperature: a temperature-jump/stopped-flow apparatus.
  • Biophys J. 2006 Dec 15;91(12):4605-10. Epub 2006 Sep 29
  • PubMed Abstract Full Text
  • Zeng W, Seward HE, Málnási-Csizmadia A, Wakelin S, Woolley RJ, Cheema GS,

    Basran J, Patel TR, Rowe AJ, Bagshaw CR.

  • Resonance energy transfer between green fluorescent protein variants: complexities revealed with myosin fusion proteins.
  • Biochemistry. 2006 Sep 5;45(35):10482-91.
  • E-mail reprint request PubMed Abstract Full Text
  • Tóth J, Gombos L, Simon Z, Medveczky P, Szilágyi L, Gráf L, Málnási-Csizmadia A.
  • Thermodynamic analysis reveals structural rearrangement during the acylation step in human trypsin 4 on 4-methylumbelliferyl 4-guanidinobenzoate substrate analogue.
  • J Biol Chem. 2006 May 5;281(18):12596-602. Epub 2006 Feb 21.
  • PubMed Abstract Full Text

2005

2004

  • Zeng W, Conibear PB, Dickens JL, Cowie RA, Wakelin S, Málnási-Csizmadia A, Bagshaw CR.
  • Dynamics of actomyosin interactions in relation to the cross-bridge cycle.
  • Philos Trans R Soc Lond B Biol Sci. 2004 Dec 29;359(1452):1843-55. Review
  • PubMed Abstract Full Text
  • Conibear PB, Málnási-Csizmadia A, Bagshaw CR.
  • The effect of F-actin on the relay helix position of myosin II, as revealed by tryptophan fluorescence, and its implications for mechanochemical coupling.
  • Biochemistry. 2004 Dec 14;43(49):15404-17.
  • E-mail reprint request PubMed Abstract Full Text
  • Nagy A, Cacciafesta P, Grama L, Kengyel A, Málnási-Csizmadia A, Kellermayer MS.
  • Differential actin binding along the PEVK domain of skeletal muscle titin.
  • J Cell Sci. 2004 Nov 15;117(Pt 24):5781-9. Epub 2004 Oct 26.
  • PubMed Abstract Full Text
  • Bódis E, Strambini GB, Gonnelli M, Málnási-Csizmadia A, Somogyi B.
  • Characterization of f-actin tryptophan phosphorescence in the presence and absence of tryptophan-free myosin motor domain.
  • Biophys J. 2004 Aug;87(2):1146-54.
  • PubMed Abstract Full Text
  • Kovács M, Tóth J, Málnási-Csizmadia A, Bagshaw CR, Nyitray L.
  • Engineering lysine reactivity as a conformational sensor in the Dictyostelium myosin II motor domain.
  • J Muscle Res Cell Motil. 2004;25(1):95-102.
  • E-mail reprint request PubMed Abstract Full Text

2003

  • Farkas L, Malnasi-Csizmadia A, Nakamura A, Kohama K, Nyitray L.
  • Localization and characterization of the inhibitory Ca2+-binding site of Physarum polycephalum myosin II.
  • J Biol Chem. 2003 Jul 25;278(30):27399-405. Epub 2003 May 16.
  • PubMed Abstract Full Text

2002

  • Wakelin S, Conibear PB, Woolley RJ, Floyd DN, Bagshaw CR, Kovács M, Málnási-Csizmadia A.
  • Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes.
  • J Muscle Res Cell Motil. 2002;23(7-8):673-83. Review.
  • E-mail reprint request PubMed Abstract Full Text
  • Kovacs M, Malnasi-Csizmadia A, Woolley RJ, Bagshaw CR.
  • Analysis of nucleotide binding to Dictyostelium myosin II motor domains containing a single tryptophan near the active site.
  • J Biol Chem. 2002 Aug 9;277(32):28459-67. Epub 2002 Apr 23.
  • PubMed Abstract Full Text

2001

  • Málnási-Csizmadia A, Pearson DS, Kovács M, Woolley RJ, Geeves MA, Bagshaw CR.
  • Kinetic resolution of a conformational transition and the ATP hydrolysis step using relaxation methods with a Dictyostelium myosin II mutant containing a single tryptophan residue.
  • Biochemistry. 2001 Oct 23;40(42):12727-37. Erratum in: Biochemistry. 2004 Nov 9;43(44):14316.
  • E-mail reprint request PubMed Abstract Full Text
  • Malnasi-Csizmadia A, Kovacs M, Woolley RJ, Botchway SW, Bagshaw CR.
  • The dynamics of the relay loop tryptophan residue in the Dictyostelium myosin motor domain and the origin of spectroscopic signals.
  • J Biol Chem. 2001 Jun 1;276(22):19483-90. Epub 2001 Feb 22.
  • PubMed Abstract Full Text

2000

  • Málnási-Csizmadia A, Woolley RJ, Bagshaw CR.
  • Resolution of conformational states of Dictyostelium myosin II motor domain using tryptophan (W501) mutants: implications for the open-closed transition identified by crystallography.
  • Biochemistry. 2000 Dec 26;39(51):16135-46.
  • E-mail reprint request PubMed Abstract Full Text

1999

1998

1996

1994

Patents

  • Development of photoinducible cross-linking reagents with affinity tag to detect and isolate protein complexes.
    Hungarian patenting in process
  • Water-soluble carotene dispersions.
    Hungarian patenting in process
  • Transgenic Caenorhabditis elegans
    PCT and Hungarian patenting in process

Conference talks, poster presentations

2013

  • A Structural Model of Actomyosin in the Prepowerstroke State: Actin Binding Induced Extra Priming of Myosin
  • Biophysical Society 57th Annual Meeting, Philadelphia
  • poster presentation by Boglárka Várkuti
  • View Attachment
  • The mechanism of effectiveness in the myosin motor
  • Biophysical Society 57th Annual Meeting, Philadelphia
  • speaker: András Málnási-Csizmadia
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2012

  • Contribution of 2D, 3D structural features of drug molecules in the prediction of Drug Profile Matching
  • ChemAxon UGM, Budapest
  • speaker: Ágnes Peragovics
  • View Attachment
  • Hogyan jósoljuk meg a gyógyszermolekulák hatásprofilját?
  • TÁMOP zárókonferencia, Dobogók?
  • speaker: Ágnes Peragovics
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  • Exploring drug induced expression patterns
  • Genetics and Chemistry Sharing a Language of Discovery, Cell Symposium
  • oral and poster presentation by Gábor Szegvári
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  • Structural Model of the Pre-Powerstroke State of the Actomyosin Complex
  • Biophysical Society 56th Annual Meeting, San Diego
  • speaker: András Málnási-Csizmadia
  • View Attachment
  • Az erőkifejtés kulcsa: a miozin aktivációs hurok
  • Budapest Science Meetup
  • speaker: Boglárka Várkuti
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  • An Actomyosin Model of the Initial State of the Powerstroke
  • European Muscle Conference, Rhodes
  • speaker: Boglárka Várkuti
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2011

  • Exploration of unidentified effects for approved drugs
  • Magyar Biokémiai Egyesület Gyógyszer-biokémiai Szakosztály Munkaértekezlete, Balatonöszöd
  • poster presentation by László Végner
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  • Effect prediction by Drug Profile Matching
  • Vienna Summer School Drug Design
  • poster presentation by Ágnes Peragovics
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  • Statistical ligand efficiencies for effect specific compound library filtering
  • 4th European Conference on Chemistry for Life Sciences, Budapest
  • poster presentation by Ágnes Peragovics
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  • Statistical ligand efficiencies for effect specific compound library filtering
  • ChemAxon UGM, Budapest
  • poster presentation by Ágnes Peragovics
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  • Relationship between internal friction and the roughness of the energy landscape of protein conformational changes
  • Biophysical Meeting, Baltimore
  • poster presentation by Anna Rauscher
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  • Az enzimek belső súrlódásának energetikai modellezése
  • TÁMOP Nyitó konferencia, Dobogók?
  • speaker: Anna Rauscher
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  • Transient kinetic study of the Mg2+ regulation of myosin
  • CamProEng Cambridge
  • speaker: Anna Rauscher
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  • DrugPredict: Online drug discovery service using polypharmacology-based interaction profiling
  • Chemaxon User Group Meeting, Budapest
  • speaker: Zoltán Simon
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  • Drug effect prediction by in silico generated protein-small molecule affinity profiles
  • 4th European Conference on Chemistry for Life Sciences, Budapest
  • speaker: Zoltán Simon
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  • A novel actin binding site of myosin is responsible for effective muscle contraction
  • Biophysical Society 55th Annual Meeting, Baltimore
  • poster presentation by Boglárka Várkuti
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  • A novel actin binding site enables effective muscle contraction
  • European Muscle Conference, Berlin
  • speaker: Boglárka Várkuti
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2010

  • Elevated rotational freedom causes greater energy dissipation
  • Prague’s Protein Spring
  • poster presentation by Anna Rauscher
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  • Temperature dependence of internal friction
  • PROTEIN STRUCTURE AND DYNAMICS, Rome
  • poster presentation by András Málnási-Csizmadia
  • View Attachment

2009

  • The molecular mechanism of actin activation of myosin
  • European Muscle Conference, Lille, France
  • poster presentation by Boglárka Várkuti
  • View Attachment

2008

  • The mechanism of the reverse recovery-step, phosphate release and actin activation
  • Joint Meeting of The Biophysical Society, Long Beach, California
  • Invited speaker: András Málnási-Csizmadia, presented by Mihály Kovács
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  • A konformációváltozás során fellépő belső súrlódás hőmérsékletfüggése
  • Biofizikai Vándorgy?lés, Szeged
  • speaker: Anna Rauscher
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  • A prolin hurok szerepe a miozin II enzimmechanizmusában
  • Magyar Biokémiai Egyesület Vándorgy?lése, Szeged
  • poster presentation by Boglárka Várkuti
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2007

  • The power stroke and the phosphate release of myosin.
  • Molecular Motors Workshop, Alpbach, Austria
  • speaker: András Málnási-Csizmadia
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  • Reversible movement of switch 1 loop of myosin determines actin interaction.
  • IV. International Conference on Molecular Recognition, Pecs, Hungary
  • Speaker: Bálint Kintses
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  • Reversible movement of switch 1 loop of myosin determines actin interaction.
  • 7th Young Scientist Forum, Vienna, Austria
  • Speaker: Bálint Kintses
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  • Reversible movement of switch 1 loop of myosin determines actin interaction.
  • IV. International Conference on Molecular Recognition, Pecs, Hungary
  • Poster presentation of Bálint Kintses
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  • Reversible movement of switch 1 loop of myosin determines actin interaction.
  • European Biophysics Congress, London, UK
  • Poster presentation of Bálint Kintses
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  • Loop 4 of myosin stabilizes actomyosin interaction in weak actin binding states.
  • European Biophysics Congress, London, UK
  • Poster presentation of Máté Gyimesi
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  • Actin directly accelerates the power stroke of Dictyostelium myosin II.
  • European Biophysics Congress, London, UK
  • Poster presentation of András Málnási-Csizmadia
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  • Enzyme kinetics above denaturation temperature: a temperature-jump/stopped-flow apparatus.
  • 7th FEBS Young Scientist Forum, Vienna, Austria
  • Poster presentation of Máté Gyimesi
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  • FRET investigation of the conformation and orientation of myosin switch-1 loop, in Hungarian
  • Biológus TDK Konferencia, ELTE
  • Poster presentation of László Végner
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2006

  • Switch 1 movement determines actin binding of myosin.
  • HHMI meeting, Janelia Farm, Washington DC
  • Speaker: András Málnási-Csizmadia
  • View Attachment
  • Internal protein friction.
  • Solid sate physics to biophysics. Croatia, Dubrovnik, invited speaker
  • Speaker: András Málnási-Csizmadia
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  • Friction, Internal viscosity, Force.
  • Physics of molecular machines, joining theory and experiments, Les Houches, France, invited speaker
  • Speaker: András Málnási-Csizmadia
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  • Switch 1 movement of myosin.
  • Biophysical Society Annual Meeting, Salt lake City
  • Speaker: András Málnási-Csizmadia
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  • Enzyme kinetics above denaturation temperature.
  • The 50th Annual Meeting of the Biophysical Society, Salt Lake City, USA
  • Poster presentation of Bálint Kintses
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  • Cardiac loop of myosin II has functional role in the weak binding of actin-myosin interaction.
  • The 50th Annual Meeting of the Biophysical Society, Salt Lake City, USA
  • Poster presentation of Máté Gyimesi
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2005

  • Switch movements of myosin.
  • European Muscle Club Conference, Hungary
  • Speaker: András Málnási-Csizmadia
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  • The influence of F-actin on myosin II conformational states as revealed by tryptophan fluorescence.
  • Budapest, FEBS
  • Speaker: András Málnási-Csizmadia  
  • View Attachment
  • Effect of actin on myosin ATPase.
  • Biophysical Society Annual Meeting, Los Angeles
  • Speaker: András Málnási-Csizmadia
  • View Attachment
  • The influence of F-actin on myosin II conformational states as revealed by tryptophan fluorescence
  • EMBO/HHMI Central European Scientists Meeting, Budapest, Hungary
  • Poster presentation of András Málnási-Csizmadia
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2004

  • Actomyosin cycle.
  • ELSO., Nizza, invited speaker
  • Speaker: András Málnási-Csizmadia
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2003

  • Probes of the myosin cleft that sense actin binding.
  • Biophysical Society Annual Meeting, San Antonio
  • Speaker: András Málnási-Csizmadia
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2002

  • Fine tuning of ATP hydrolysis rate by subdomain interaction in myosin
  • Biophysical Society Annual Meeting, San Francisco
  • Speaker: András Málnási-Csizmadia
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2001

  • Kinetic separation of the open-closed transition and the ATP hydrolysis step in myosin.
  • London Muscle Series, National Research of Medical Institute, Medical Reseach Council
  • Speaker: András Málnási-Csizmadia
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  • Open-Closed transition is separate from ATP hydrolysis
  • European Muscle Conference, Pavia
  • Speaker: András Málnási-Csizmadia
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1998

  • Ca-binding of the myosin regulatory domain.
  • Dept of Biology, Brandeis University, Boston
  • Speaker: András Málnási-Csizmadia
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1997

  • Myosin regulatory domain
  • Workshop of the Hungarian Biochemical Society
  • Speaker: András Málnási-Csizmadia
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1996

  • Study of the myosin regulation with recombinant proteins: mutants, chimeras and hybrids.
  • Workshop of the Hungarian Biochemical Society
  • Speaker: András Málnási-Csizmadia
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